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As shown in Figure 4, equally undifferentiated and differentiated SMCs and ASCs were being dissociated into separate, semi-adherent cells when the culture medium was changed using a non-enzymatic mobile dissociation buffer at 37 °C for thirty min. This permitted the cells to deal while remaining in contact with the rigid plastic surface of your lifestyle properly. determine 4(A1,A2) exhibits the sub-confluence and comfortable point out of differentiated SMCs just before detachment and contraction. By contrast, determine four(A3–A5) demonstrates a reduce of cell dimensions at various time points after cure with carbachol. When treated with 100 µM carbachol, the SMCs started off contraction quickly, as demonstrated in determine four(A3).
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